Villagers fear plans to fell up to eight trees to create an extra road lane will increase traffic and make the area more dangerous.
Proposals by Gladman Developments to alter Queniborough crossroads – including removing trees in Rearsby Road – are part of an application to build 165 houses off Barkby Road.
A consultation into the scheme by Charnwood Borough Council runs until September 11.
Opponents of the plans have tied tape around the trunks of the threatened trees and put up signs which say: "No to houses. No to slip road."
Amanda Grant, 35, who lives directly opposite the trees, said she was told about the proposal last Thursday by Queniborough Parish Council, which forwarded a message from Charnwood Borough Council.
Amanda said as a resident and mum of two young children, she was worried about increased pollution, noise levels and vehicle numbers.
She said: "It is such a busy road from all directions.
"It is going to make the traffic heavier and faster-flowing through the village. It will be a rat run.
"The trees have been there for years. They make the village aesthetically pleasing."
Amanda said she hoped to arrange a meeting with neighbours to discuss the matter.
"We want to get as many people as possible to object. We feel as if we have very little time.
"I think as a parish we need to come together."
Anne Agger, 75, who also lives opposite the trees, said: "The trees help to protect us from the pollution and we are already inundated with traffic.
"Putting in a slip road is not going to improve anything. It is just going to make it worse for children and villagers.
"We are very worried about children going to the school."
Anne, who has lived in the village with her husband for 47 years, said the trees that may be removed included limes, pines and horse chestnuts.
Daniel Grimley, borough councillor for Queniborough, said: "I would urge residents to write to Charnwood Borough Council planning department." He said residents could also raise issues with him.
"I am concerned about anything that would alter the character of the village and I know the crossroads does get fairly busy," he said.
A council spokesman said: "All planning applications are judged on their merit.
"Additional information has been submitted by the applicants to reduce the potential impact of the scheme on the main road.
"We have decided to extend the consultation on this application for another 21 days, to ensure that residents are able to comment on this new information.
"Consultation is now open until September 11, and the application is due to be considered by the plans committee in October or November."
People who would like to express their opinion about the plans should e-mail Charnwood Borough Council on:
development.control@ charnwood.gov.uk
Contact Councillor Grimley on:
cllr.daniel.grimley@ charnwood.gov.uk
